What QC metrics matter most for your custom siRNA Synthesis oligos?

When you order custom siRNA from Synbio Technologies, quality control (QC) is critical for ensuring that the oligos will perform reliably for Gene Silencing experiments. Here we outline which QC metrics matter most — and why they influence the success of your experiments.

Purity and Identity Verification

One of the most important QC metrics is the purity of the siRNA oligos. Synbio Technologies purifies its siRNA products using HPLC (HighPerformance Liquid Chromatography) (or OPC) to remove truncated or incomplete synthesis products.
In addition to purification, correct sequence identity is essential. Synbio confirms the molecular integrity of RNA oligos by mass analysis (MS) as part of QC reporting.
High purity and confirmed identity ensure that the siRNA duplex corresponds exactly to the designed sequence, minimizing unwanted by-products or degradation, both of which can compromise downstream Gene Silencing efficiency.

Duplex Integrity and Contamination Control

For siRNA to function, both sense and antisense strands must be properly synthesized and annealed into a duplex form. Synbio’s QC includes analysis that verifies purity and correct duplex formation, which helps guarantee that the delivered siRNA will behave as expected in cellular assays.
Another critical metric is the avoidance of contamination — in particular RNase contamination, which can degrade RNA rapidly and render siRNA nonfunctional. Synbio applies stringent RNasefree practices during synthesis, purification, and storage to avoid degradation.

Yield and Consistency

While purity and identity are fundamental, the yield and reproducibility of production also matter. Synbio offers siRNA in a standard yield (e.g. 2.5 nmol for nonmodified siRNA) and provides a QC Report (COA) plus a QC Report including MS/HPLC data with each batch.
Consistent yield and batchtobatch reproducibility are important for planning experiments, ensuring that users have sufficient material for transfection or functional assays without unexpected variation in concentration or quality.

Functional Performance: Guaranteed Silencing Efficiency

Beyond chemical and physical QC metrics, the ultimate test of custom siRNA quality is functional performance. Synbio offers a “guaranteed siRNA service”: when they design and provide three or four candidate siRNAs, at least one is expected to effectively inhibit the target gene, with a knockdown (silencing) efficiency of at least 70%, given transfection efficiency of at least 90%.
This functional guarantee matters because even chemically perfect siRNA might fail to silence the target if its sequence or structure is sub-optimal. Thus, design, synthesis, QC, and biological testing together define the usability of custom siRNA for Gene Silencing.

Conclusion

In summary, when using Synbio Technologies for custom siRNA Synthesis, the QC metrics that matter most are: (1) purity (via HPLC), (2) correct sequence identity (via mass analysis), (3) duplex integrity and RNasefree handling, (4) consistent yield and batch reproducibility, and (5) functional performance (silencing efficiency), especially when the “guaranteed siRNA” service is used. Additionally, if chemical modifications are chosen, verification of modification and maintained purity are critical. By paying attention to these metrics, users maximize the likelihood that their Gene Silencing experiments will be effective and reproducible.